Toxicological studies of additives feed endoforce multi on laboratory animals

The creation of new drugs and feed additives is an urgent task that contributes to the growth efficiency livestock poultry industries agriculture, especially during period import substitution in Russian Federation. An experimental study possible changes body laboratory animals after single or multiple uses necessary for toxicological assessment newly created products. Single intragastric administration additive Endoforce Multi containing live vegetative cells spores cultures: Bacillus subtilis B-5449, B-1323, B-3679 - 5.0 109 CFU / ml, at a dose many times higher than recommended, did not lead death animals, which confirmed absence its acute toxicity. Over entire observation period, authors note any disturbances rats’ behavioural reactions physiological states. Determination chronic toxicity was carried out 30 days. studied suspension given with water rats groups doses: 1st 100 mg, 2nd 150 mg 3rd 200 per head. At end empirical study, increase number erythrocytes by 2.68–3.72 1012/l, haemoglobin 41.50–61.58 g/l compared control ones revealed, indicated blood oxygenation organism as whole. In addition, groups, decrease content urea 3.14 ± 0.53 4.68 0.41 mmol l creatinine 32.26 5.18 48.13 6.23 µmol/l. opposite trend observed when determining serum activity alkaline phosphatase, increased 39.0–52.6% intact individuals, dynamic processes growth, formation development skeleton. According results autopsy regardless introduction Multi, no pathological colour, consistency size internal organs were registered.